Cbs-scientific QNX-700 Manual do Utilizador

Quadra Electrophoresis Systems4-Place Mini-Vertical Slab Gel /Blotting SystemsQNC-700, with coolingQNX-700INSTRUCTION MANUAL

10Quadra Instructions 9-30-133.2 Running the Gel1. Place stirring bar into bottom of reservoir in stirring corral.2. Place core assemblies into lower

11www.cbsscientic.com793.3 Removing the Gel1. Turn the power supply off and discon-nect the leads from the power supply. Remove the safety cover fro

12Quadra Instructions 9-30-134.1 Alternate Protocol: Using Gel Wrap™ Gasket Casting Method (Not using Pre-cast gels)1. Place all components in an a

13www.cbsscientic.com4.1 Alternate Protocol: Using Gel Wrap™ Gasket Casting Method (Not using Pre-cast gels)1. Place all components in an authoriz

14Quadra Instructions 9-30-13SECTION 4 Alternate Protocol for Slab Gel Electrophoresis. (Not using Pre-cast gels)4.2 Preparing the Electrophoresis U

15www.cbsscientic.com1SECTION 5 Instructions for Western Blotting5.1 Preparing the Unit for Blotting1. Remove safety cover from the assembled unit b

16Quadra Instructions 9-30-134. Insert blotting casettes into core making sure that red side faces outward. See diagram 5.5. Close doors and re-latch

17www.cbsscientic.com4. Insert blotting casettes into core making sure that red side faces outward. See diagram 5.5. Close doors and re-latch by pres

18Quadra Instructions 9-30-136.2 Electro-Blotting6.3 References1. Hames, B.D. (ed.) (1998). Gel Electrophoresis of Proteins. A Practical Approach.

19www.cbsscientic.comAs a general recommendation, equilibrate gels (after running) with the diluted transfer buffer for 5 to 10 minutes before transf

2Quadra Instructions 9-30-13TABLE OF CONTENTS Important User Information . . . . . . .

20Quadra Instructions 9-30-136.4 Recommended ClearPAGE™ Buffer Formulations, con’t.LDS Sample Buffer, 4x, (Cat. # FB31010) *pH should be betwee

21www.cbsscientic.comSECTION 7Maintenance of Equipment7.1 Care and Handling The plastic components of the Quadra Electrophoresis Systems are fabrica

22Quadra Instructions 9-30-13SECTION 8Ordering Information CAT. # DESCRIPTIONQUADRA COMBO MINI-VERTICALS - 10CM(W) X 10CM(H)*QNX-700Quadra System

23www.cbsscientic.comSECTION 8Ordering InformationClearPAGE SDS gels, packages of 10 - Cat. #’sAcrylamide %Gel Size (cm) W x H1 wellCat. #2D well tro

CONTACT INFORMATIONOnline Ordering www.cbsscientic.comTelephone Local and International: 858-755-4959 Toll Free: 800-243-4959Sales E-mail Address

3www.cbsscientic.comIMPORTANT USER INFORMATIONThis Instruction Manual will explain how to use this product safely and effectively. Please read and c

4Quadra Instructions 9-30-13FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURSLe présent manuel d’utilisation explique la manière de se serv

5www.cbsscientic.comSECTION 1General Information 1.1 IntroductionC.B.S. Scientic offers the Quadra Electrophoresis Systems for performing SDS-Pag

6Quadra Instructions 9-30-131.3 Comb Specications for Hand Cast Gels1.4 SafetyPower to the Quadra Electrophoresis System is to be supplied by an

7www.cbsscientic.comSECTION 2 Description of Parts2.1 Unpacking and Components Please verify that your unit comes complete with the following comp

8Quadra Instructions 9-30-13SECTION 3 Instructions for Slab Gel Electrophoresis Using Pre-Cast Gels3.1 Preparing the Electrophoresis Unit1. Power le

9www.cbsscientic.comSECTION 3 Instructions for Slab Gel Electrophoresis Using Pre-Cast Gels3.1 Preparing the Electrophoresis Unit1. Power leads are