Cbs-scientific DGGEK-4801 Manual do Utilizador

Cipher DGGE Electrophoresis SystemsDGGEK-4001 DGGEK-4801 INSTRUCTION MANUAL

18 19www.cbsscientic.comCipher DGGE Instructions 2/28/143.2 Gel Casting Techniques- continuedB. Vertical gradient gel casting using GM-40 gradient m

20 21www.cbsscientic.comCipher DGGE Instructions 2/28/143.2 Gel Casting Techniques- continued D. Vertical gradient gel casting using GM-40 gradien

22 23www.cbsscientic.comCipher DGGE Instructions 2/28/143.5 Perpendicular Gradient FormationTo determine the range of perpendicular gradient approp

24 25www.cbsscientic.comCipher DGGE Instructions 2/28/14SECTION 4 Gel Running Preparations4.1 Preparation of the Cassettes1. After polymerization

26 27www.cbsscientic.comCipher DGGE Instructions 2/28/144.2 Buffer Cycling Connections-continued.Buffer Cycling Connections – Dual Cassettes

28 29www.cbsscientic.comCipher DGGE Instructions 2/28/14SECTION 6Recommended Buffers and Reagents Warning: Do not mix buffer i

30 31www.cbsscientic.comCipher DGGE Instructions 2/28/14SECTION 8 References1. S.G. Fischer and L.S. Lerman, (1983) PNAS 80:1579.2. R.M. Myers, T.

32 33www.cbsscientic.comCipher DGGE Instructions 2/28/14SECTION 10Maintenance of Equipment10.1 Care and HandlingThe plastic components of the D

34 35www.cbsscientic.comCipher DGGE Instructions 2/28/14  13R, 712, 7306, 7312, 812, 8006, 8012, 8002, 8112, 8102, 8106, 8202, 8212,

CONTACT INFORMATIONOnline Ordering www.cbsscientic.comTelephone Local and International: 858-755-4959 Toll Free: 800-243-4959Sales E-mail Address

2 3www.cbsscientic.comCipher DGGE Instructions 2/28/14IMPORTANT USER INFORMATIONThis Instruction Manual will explain how to use this product safely a

4 5www.cbsscientic.comCipher DGGE Instructions 2/28/14FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURSLe présent manuel d’utilisation exp

6 7www.cbsscientic.comCipher DGGE Instructions 2/28/14 1.3 SafetyPower to the DGGE systems is to be supplied by an external DC voltage power suppl

8 9www.cbsscientic.comCipher DGGE Instructions 2/28/14   Front View Re

10 11www.cbsscientic.comCipher DGGE Instructions 2/28/142.3.4 Controller Operation 2.3.4.a Reservoir Liquid Level Fill the reservoir with the

12 13www.cbsscientic.comCipher DGGE Instructions 2/28/14 2.3.4.h Local Lockout FeatureThis feature enables the user to lock all controls on

14 15www.cbsscientic.comCipher DGGE Instructions 2/28/14To mains (wall outlet)To mains (wall outlet)Figure 2-2abcdPower Supply ConnectionDGGEK Unit S

16 17www.cbsscientic.comCipher DGGE Instructions 2/28/14SECTION 3Gel Casting Options3.1 Preparation/Cleaning of Glass Plate for Gel CastingHand wash